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KMID : 0545120010110020342
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Journal of Microbiology and Biotechnology
2001 Volume.11 No. 2 p.342 ~ p.345
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Enzymatic Degradation of Poly(γ-glutamic acid) Hydrogel Prepared by γ-Ray Irradiation
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HARA, TOSHO
CHOI, SEONG-HYUN/CHOI, WOO-YOUNG
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Abstract
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A bacterial strain PHA which produces an enzyme catalyzing the degradation of crosslinked poly(γ-glutamic acid) hydrogels, was isolated and identified as a Flavobacterium sp. The enzyme was obtained by the sonication of the bacterial cells preincubated in a Bouillon medium with shaking, without adding of poly(γ-glutamic acid) as an inducer. The products of the hydrogel degraded by the crude enzyme agreed closely with the depolymerized materials in SDS-polyacrylamide gel electrophoresis using methylene blue staining, and with a glutamic acid monomer on thin-layer chromatography, thereby suggesting that strain PH-4 produced a kind of exohydrolase.
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